Influenza A virus production in a single-use orbital shaken bioreactor with ATF or TFF perfusion systems
نویسندگان
چکیده
منابع مشابه
Study of a recombinant CHO cell line producing a monoclonal antibody by ATF or TFF external filter perfusion in a WAVE Bioreactor™
Background Major advantages of perfusion are high cell numbers and high total production in a relatively small size bioreactor. Moreover, perfusion is optimal when the product of interest is unstable or if the product yield is low. On the other hand, disadvantages are for example technical challenges originating from non-robust cell separation devices as well as sterility concerns from the more...
متن کاملVery High Density of CHO Cells in Perfusion by ATF or TFF in WAVE Bioreactor™. Part I. Effect of the Cell Density on the Process
High cell density perfusion process of antibody producing CHO cells was developed in disposable WAVE Bioreactor™ using external hollow fiber filter as cell separation device. Both "classical" tangential flow filtration (TFF) and alternating tangential flow system (ATF) equipment were used and compared. Consistency of both TFF- and ATF-based cultures was shown at 20-35 × 10(6) cells/mL density s...
متن کاملVery High Density of Chinese Hamster Ovary Cells in Perfusion by Alternating Tangential Flow or Tangential Flow Filtration in WAVE Bioreactor™—Part II: Applications for Antibody Production and Cryopreservation
A high cell density perfusion process of monoclonal antibody (MAb) producing Chinese hamster ovary (CHO) cells was developed in disposable WAVE Bioreactor™ using external hollow fiber (HF) filter as cell separation device. Tangential flow filtration (TFF) and alternating tangential flow (ATF) systems were compared and process applications of high cell density perfusion were studied here: MAb pr...
متن کاملStudy of a recombinant CHO cell line producing a monoclonal antibody by ATF or TFF external filter perfusion in a WAVE BioreactorTM
Background Major advantages of perfusion are high cell numbers and high total production in a relatively small size bioreactor. Moreover, perfusion is optimal when the product of interest is unstable or if the product yield is low. On the other hand, disadvantages are for example technical challenges originating from non-robust cell separation devices as well as sterility concerns from the more...
متن کاملoptimization of operational parameters in rhamnolipid production by pseudomonas aeruginosa mm1011 in a miniaturized shaken bioreactor
a rhamnolipid–type biosurfactant production by pseudomonas aeruginosa mm1011 was studied in a miniaturized shaken bioreactor. the operational parameters, affecting the biosurfactant production in shaken bioreactors, such as shaking frequency (200, 250, 300 rpm), filling volume (50, 75, 100 ml), and aeration rate (0.2, 0.6, 1 vvm), were optimized using response surface methodology. the optimizat...
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ژورنال
عنوان ژورنال: Vaccine
سال: 2019
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2019.06.005